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KMID : 1098420090170030210
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 3 p.210 ~ p.216
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Improvement of Immune Activities of Rhodiola sachalinensis A. Bor. by Serial Solvent Fractionization
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Ha Ji-Hye
Kim Cheol-Hee
Jeong Hyang-Suk
Kim Young
Oh Sung-Ho
Jeong Myeong-Hoon
Ma Choon-gje
Nam Jong-Hyeon
Hwang Baik
Choi Geun-Pyo
Park Uk-Yeon
Lee Hyeon-Yong
Kim Seung-Seop
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Abstract
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This study was performed to compare the effects of immuno-modulating activities of Rhodiola sachalinensis A. Bor. fractionized by consecutive solvent separation. The Cytotoxicity of all fractionized extracts on human kidney cell (HEK293) was lower than crude extracts. Generally, the butanol and chloroform extracts showed less cytotoxicity on about 10.07% and 9.67% than the crude extracts. For human immune B and T cell growth, chloroform fraction showed the highest cell growth compared to the control. The secretion of cytokines (IL-6, TNF-α) on human B and T cells were increased by adding chloroform extracts. Also, NK cell growth was significantly improved up to nearly 30% by adding the supernatant of
B cell medium grown with the chloroform fraction. It was also found that chloroform fraction could yield higher nitric oxide
production from macrophage than untreated control cells. Differentiation of HL-60 cells was increased up to 131.9% after
treatment with chloroform fraction extracts, compared to the control. These results indicate that the chloroform fraction of
R. sachalinensis have high immune activation activity than others fractions and the crude extracts, implying that this chloroform fractions could be used a new functional material.
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KEYWORD
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odiola sachalinensis A. Bor., Consecutive Solvent Fraction, Immune Activation Activity, Natural Killer (NK) Cell
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